Mouse Leukotriene A4 Hydrolase ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit from Antibodies.com is intended for the in vitro quantitative determination of mouse Leukotriene A4 Hydrolase (LTA4H) in serum, plasma, tissue homogenates or other biological fluids. The assay employs the sandwich immunoassay technique using a pre-coated 96-well microplate arranged as 12 x 8 well strips, with detection via a biotinylated anti-LTA4H antibody, HRP-avidin conjugate and TMB substrate, read at 450 nm on a colorimetric microplate reader.

Performance specifications include a product range of 0.156-10 ng/ml and a sensitivity of <0.051 ng/ml. The total assay time is approximately 4h 30m. Recovery has been demonstrated in serum (83-100%, average 91%), EDTA plasma (91-98%, average 94%) and heparin plasma (90-99%, average 94%), with dilution linearity reported at 1:2, 1:4, 1:8 and 1:16 across the same sample types.

Kit components comprise a pre-coated 96-well microplate, lyophilized standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution and plate sealers, with storage temperatures varying between +4°C and -20°C depending on the component.