Mouse Leukocyte Cell Derived Chemotaxin 1 ELISA Kit

This Sandwich ELISA kit from Antibodies.com enables in vitro quantitative determination of mouse LECT1 (Leukocyte Cell Derived Chemotaxin 1) in serum, plasma, or other biological fluids. The assay uses a sandwich enzyme immunoassay format on a pre-coated 96-well microplate, employing biotinylated anti-LECT1 antibody, HRP-avidin conjugate, and TMB substrate with colorimetric detection read at 450 nm using a microplate reader.

The quantitative assay range is 31.2-2000 pg/ml with sensitivity of 11.3 pg/ml, and total assay time is 4 hours 30 minutes. Kit components include a pre-coated microplate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, stored at +4°C or -20°C depending on the component.

Validation data show recovery in serum (80-102%, average 91%), EDTA plasma (81-100%, average 90%), and heparin plasma (80-89%, average 84%). Linearity at sample dilutions of 1:2, 1:4, 1:8, and 1:16 ranges from 74-108% across serum and plasma samples.