Mouse Lectin Like Oxidized Low Density Lipoprotein Receptor 1 ELISA Kit

From Antibodies.com, this Mouse LOX 1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) intended for the in vitro quantitative determination of mouse LOX 1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1) in serum, plasma, tissue homogenates, or other biological fluids. The assay is reactive with Mouse samples and is built around a pre-coated 96-well microplate arranged as 12 x 8 well strips, with absorbance read on a colorimetric microplate reader at 450 nm.

The kit has a stated assay range of 12.5-800 pg/ml and a sensitivity of <5.1 pg/ml, with a total assay time of 4h 30m. Published spiked-recovery figures give an average of 91% (range 80%-102%) for serum, 90% (81%-100%) for EDTA plasma, and 84% (80%-89%) for heparin plasma across n=5 for each matrix, together with dilution linearity data at 1:2, 1:4, 1:8, and 1:16 dilutions.

Detection uses a Biotinylated Anti-LOX 1 Antibody followed by an HRP-Avidin conjugate; TMB substrate is added to generate a blue product that turns yellow after the acidic stop solution, with yellow intensity proportional to LOX 1 concentration in each well.