Mouse Kidney Injury Molecule 1 ELISA Kit

A sandwich-format ELISA from Antibodies.com for the in vitro quantitative determination of mouse Kidney Injury Molecule 1 (TIM 1) in tissue homogenates, urine, cell culture supernates, and other biological fluids. The assay employs a pre-coated 96-well microplate (12 x 8 well strips), a biotinylated anti-TIM 1 detection antibody, an HRP-avidin conjugate, and TMB substrate; the enzymatic reaction is read at 450 nm on a colorimetric microplate reader, with yellow colour intensity proportional to the TIM 1 concentration calculated against the standard curve.

Total assay time is 4h 30m, with a measuring range of 39-2500 pg/ml and a sensitivity of 16 pg/ml. Kit components are stored at +4°C or -20°C as instructed, and the kit is indicated for use with tissue homogenates, cell culture supernates and urine.