Mouse Jagged 1 Protein ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is designed for the in vitro quantitative determination of mouse Jagged1 in tissue homogenates or other biological fluids. The kit employs a pre-coated 96-well microplate arranged as 12 x 8 well strips, and uses a biotinylated anti-Jagged1 detection antibody with HRP-Avidin conjugate visualised via TMB substrate, producing a yellow colour measured at 450 nm on a colorimetric microplate reader.

The assay has a quantitative working range of 0.156–10 ng/ml with a sensitivity of 0.064 ng/ml and a total run time of 4 hours 30 minutes. Validation data report average spike-and-recovery values of 91% for serum, 90% for EDTA plasma, and 84% for heparin plasma, with linearity data provided at dilutions from 1:2 to 1:16 across these sample types.