Mouse Interleukin-5 ELISA Kit

A 192-well format microplate-based immunoassay from Antibodies.com enables quantitative detection of mouse Interleukin-5 (IL-5), a Th2-associated cytokine central to eosinophil proliferation, activation, and survival, as well as B-cell class switching to IgA. Immunology and respiratory disease laboratories use this kit to profile cytokine responses in murine models of allergic airway inflammation, helminth infection, parasite challenge, and asthma research.

The kit provides 192 tests in a single plate configuration, sufficient for full standard curves run in duplicate alongside experimental and control samples. Each kit includes pre-coated capture antibody wells, a biotinylated detection reagent, streptavidin-HRP conjugate, and recombinant mouse IL-5 standard. Typical protocols call for 50 to 100 µL of diluted standard or sample per well, with absorbance readout on standard laboratory plate readers.

This assay supports analysis of cell culture supernatants from stimulated splenocyte cultures, bronchoalveolar lavage fluid, and tissue homogenates from in vivo studies. The format accommodates kinetic and endpoint readouts. Lot-specific calibration data and recommended dilution factors ship with each kit. Researchers working with limited or precious samples benefit from the kit's compatibility with frozen aliquots, which preserve analyte integrity through repeated freeze-thaw cycles when handled according to the included protocol.