Mouse Interleukin 4 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com is designed for the in vitro quantitative determination of mouse Interleukin 4 (IL-4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The assay employs a 96-well microtiter plate pre-coated with an antibody specific for IL-4. Standards and test samples are added to the wells, followed by a Biotinylated Anti-IL-4 Antibody and an HRP-Avidin conjugate. TMB substrate is then used to visualise the enzymatic reaction, producing a colour change read as absorbance at 450 nm in a colorimetric microplate reader. Total assay time is 4 hours 30 minutes. The product falls within the Assays and Kits category.

The reported product range is 31.2–2000 pg/ml with a sensitivity of <12.5 pg/ml. Recovery data (n=5) shows averages of 91% in serum (range 80%–102%), 90% in EDTA plasma (range 81%–100%), and 84% in heparin plasma (range 80%–89%). Linearity at dilutions of 1:2, 1:4, 1:8 and 1:16 (n=5) is 87–91%, 87–107%, 74–101% and 92–97% for serum; 90–105%, 84–101%, 90–101% and 79–108% for EDTA plasma; and 84–95%, 92–105%, 82–105% and 89–91% for heparin plasma. Kit components should be stored at +4°C or −20°C as instructed, and the kit must not be used past its expiration date.