Mouse Interleukin 22 Receptor Alpha 2 ELISA Kit

A sandwich ELISA kit from Antibodies.com is intended for the in vitro quantitative determination of mouse IL-22 RA2/IL-22BP (Interleukin 22 Receptor Alpha 2) in serum, plasma, tissue homogenates or other biological fluids. The assay employs a pre-coated 96-well microplate (12 x 8 well strips) with a biotinylated detection antibody and HRP-avidin conjugate, with signal developed using TMB substrate and read as absorbance at 450 nm on a colorimetric microplate reader.

According to the manufacturer, the quantitative sandwich ELISA has a working range of 0.312 to 20 ng/ml and a sensitivity of 0.119 ng/ml, with a total assay time of 4 h 30 min. Reported neat-sample recovery averages are 91% for serum (n=5, range 80 to 102%), 91% for EDTA plasma (n=5, range 81 to 101%) and 84% for heparin plasma (n=5, range 80 to 89%), with dilution linearity demonstrated from 1:2 to 1:16 across the same sample types. Kit components are stored at +4 °C or −20 °C as indicated.