Mouse Interleukin 2 Receptor Alpha ELISA Kit

Manufactured for Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of mouse IL-2 Receptor alpha in serum, plasma, tissue homogenates or other biological fluids. The assay employs a pre-coated microplate platform with colorimetric detection read at 450 nm using a microplate reader, with a total assay time of 4 hours 30 minutes.

The reported sensitivity is 0.059 ng/ml with a measuring range of 0.156-10 ng/ml. The procedure uses a biotinylated detection antibody and HRP-avidin conjugate with TMB substrate, producing a yellow product whose intensity is proportional to the target concentration. Recovery has been validated in serum (80-102%, average 91%), EDTA plasma (81-100%, average 90%) and heparin plasma (80-89%, average 84%). Kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date.