Mouse Interleukin-2 ELISA Kit

Quantitative mouse IL-2 measurement is the purpose of this Antibodies.com cytokine immunoassay, which delivers a pre-coated 96-test microplate, lyophilized recombinant IL-2 standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, and TMB substrate, enough to quantify unknowns against a full standard curve in duplicate.

Interleukin-2 is a 15.5 kDa four-alpha-helix cytokine secreted principally by activated CD4+ T lymphocytes, and it acts as the central driver of T-cell clonal expansion, regulatory T-cell differentiation, and effector lymphocyte survival. Tracking IL-2 release is a standard readout in transplantation tolerance studies, vaccine adjuvant development, autoimmune disease models, infectious disease immunology, and tumor microenvironment characterization, where stimulated splenocyte culture supernatant, draining lymph node homogenate, and tissue culture supernatant are common specimen types.

Researchers studying T-cell activation, Th1 versus Th2 versus Th17 polarization, immune checkpoint blockade, and graft-versus-host biology can monitor IL-2 dynamics across cell culture experiments and in vivo challenge studies. Reagents should be kept refrigerated between runs, with the standard reconstituted immediately before use and any unused diluted standard frozen for later experiments.