Mouse Interleukin 11 Receptor Alpha ELISA Kit

Designed for the in vitro quantitative determination of mouse Interleukin 11 Receptor Alpha (IL-11RA), this sandwich ELISA from Antibodies.com is intended for use with tissue homogenates and other biological fluids. The quantitative assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, onto which an antibody specific for IL-11RA has been immobilised. Standards and test samples are added to the wells, the plate is washed, and detection is achieved with a Biotinylated Anti-IL-11RA Antibody followed by an HRP-Avidin conjugate. A TMB substrate solution produces a blue colour that changes to yellow upon addition of acidic stop solution; absorbance is read at 450 nm and the IL-11RA concentration calculated by reference to a standard curve.

The assay has a reported sensitivity of 0.061 ng/ml and a measuring range of 0.156-10 ng/ml, with a total run time of approximately 4 hours 30 minutes. Detection is colourimetric and is performed on a microplate reader. Validation data report average recovery of 91% in serum (range 80%-102%, n=5), 91% in EDTA plasma (range 81%-101%, n=5) and 84% in heparin plasma (range 80%-89%, n=5), with dilution linearity assessed at 1:2, 1:4, 1:8 and 1:16 dilutions across each sample type. Components supplied include the pre-coated 96-well microplate, lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers, stored at +4°C or -20°C as indicated. The kit should not be used past its expiration date.