Mouse Interleukin-1 alpha ELISA Kit

This immunoassay from Antibodies.com is configured to quantify mouse Interleukin-1α in cell culture supernatants, tissue homogenates, and similar experimental matrices using a plate-based ELISA workflow. The kit ships as a complete package containing pre-coated microplate wells, biotinylated detection reagent, enzyme conjugate, wash buffer, substrate solution, and stop reagent, supporting in vitro research applications.

Interleukin-1α is a pro-inflammatory cytokine released by activated macrophages, monocytes, keratinocytes, and various epithelial cell types, mediating fever, leukocyte recruitment, and acute-phase inflammatory signaling. Quantitative measurement of IL-1α in experimental samples aids characterization of cytokine responses in mouse models of infection, autoimmunity, sterile inflammation, and tissue injury, complementing parallel readouts such as IL-1β, TNF-α, and IL-6.

Each microplate supports up to 96 determinations in a single run and includes a recombinant mouse IL-1α standard for curve generation. Researchers dilute standards and unknown samples in the provided assay buffer, add detection reagent, and develop the plate for absorbance readout. Unopened kit components should be refrigerated until first use, and reconstituted working solutions require handling conditions detailed in the product manual.

This kit is intended for research use only and is not validated for diagnostic or clinical applications.