Mouse Interferon Regulatory Factor 5 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of mouse Interferon Regulatory Factor 5 (IRF5) in serum, plasma, tissue homogenates or other biological fluids. The assay employs the sandwich enzyme immunoassay technique, using a pre-coated microplate (12 x 8 well strips) with an antibody specific for IRF5. Standards and samples are added to the wells, followed by a Biotinylated Anti-IRF5 Antibody and HRP-Avidin conjugate. TMB substrate is then used to visualize the reaction, with absorbance read at 450 nm on a colorimetric microplate reader.

The kit offers an assay range of 0.156-10 ng/ml with a sensitivity of <0.048 ng/ml, and a total assay time of 4h 30m. Reported recovery averages are 91% for serum, 90% for EDTA plasma, and 84% for heparin plasma, with dilution linearity data provided for serum, EDTA plasma, and heparin plasma across dilutions of 1:2, 1:4, 1:8, and 1:16. Components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.