Mouse Integrin Alpha 3 ELISA Kit

From Antibodies.com, this Mouse Integrin Alpha 3 ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) designed for the in vitro quantitative determination of mouse Integrin alpha 3 in tissue homogenates or other biological fluids. The assay is run on a pre-coated 96-well microplate (12 x 8 well strips); standards and samples are added to the wells, captured by an immobilized antibody specific for Integrin alpha 3, and detected using a Biotinylated Anti-Integrin alpha 3 Antibody followed by an HRP-Avidin conjugate. Signal is developed with TMB substrate and read at 450 nm on a colorimetric microplate reader.

The assay has a stated sensitivity of <0.118 ng/ml and a working range of 0.312–20 ng/ml, with a total assay time of 4h 30m. Reported average recovery values across sample types include serum (80–102%, average 91%), EDTA plasma (81–100%, average 90%), and heparin plasma (80–89%, average 84%). Kit components supplied are a pre-coated 96 well microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, each with a specified storage temperature.

Kit components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.