Mouse Insulin Like Growth Factor 2 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com, intended for the in vitro quantitative determination of mouse Insulin Like Growth Factor 2 (IGF2) in serum, plasma or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips), a biotinylated anti-IGF2 antibody, an HRP-avidin conjugate, and TMB substrate read at 450 nm.

Reported sensitivity is <42.4 pg/ml and the assay range is 93.8-6000 pg/ml. The protocol runs approximately 4h 30m, with components stored at +4°C or -20°C as instructed and not to be used past the expiration date.

Average recovery values are reported as 91% for serum (n=5, range 80%-102%), 90% for EDTA plasma (n=5, range 81%-100%), and 84% for heparin plasma (n=5, range 80%-89%). Linearity data is provided across dilutions of 1:2, 1:4, 1:8, and 1:16 for serum, EDTA plasma, and heparin plasma samples.