Mouse Inositol Polyphosphate-4-Phosphatase Type I 107kDa ELISA Kit

Antibodies.com provides a sandwich ELISA kit designed for the in vitro quantitative determination of mouse INPP4A (Inositol Polyphosphate-4-Phosphatase Type I, 107kDa) in tissue homogenates, cell lysates or other biological fluids. The assay is reactive with mouse samples and is read on a colorimetric microplate reader.

The reported assay range is 0.156-10 ng/ml with a sensitivity of <0.051 ng/ml, and the total assay time is 4h 30m. The kit employs the sandwich enzyme immunoassay technique: an antibody specific for INPP4A is pre-coated onto a 96-well microplate, standards and samples are added and any INPP4A present is captured, and bound analyte is detected with a Biotinylated Anti-INPP4A Antibody followed by an HRP-Avidin conjugate. TMB substrate is used to visualise the HRP enzymatic reaction, producing a blue product that changes to yellow after addition of an acidic stop solution; absorbance at 450 nm is read against a standard curve to determine concentration.

Kit components include a pre-coated microplate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers. Reported average recovery values are 91% for serum (range 83-100%, n=5), 95% for EDTA plasma (range 91-99%, n=5) and 94% for heparin plasma (range 90-99%, n=5). Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.