Mouse Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta ELISA Kit

Antibodies.com provides a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) for the in vitro quantitative determination of mouse IKK beta (Inhibitor of Kappa-Light Polypeptide Gene Enhancer in B-Cells Kinase Beta) in serum, plasma, tissue homogenates, cell lysates, and other biological fluids.

The kit uses a 96-well microtiter plate pre-coated with an antibody specific for IKK beta. Standards and samples are added; captured IKK beta is then detected with a biotinylated anti-IKK beta antibody, an HRP-Avidin conjugate, and TMB substrate. Absorbance is read at 450 nm. The assay has a working range of 0.156–10 ng/ml, a sensitivity of <0.054 ng/ml, and a total run time of 4 hours 30 minutes.

Average recovery across sample types is reported as 91% for serum (range 80–102%), 90% for EDTA plasma (range 81–100%), and 84% for heparin plasma (range 80–89%). Kit components are stored at +4°C or −20°C as instructed in the protocol, and the kit should not be used past its expiration date.