Mouse Inhibin A ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is designed for the in vitro quantitative determination of mouse Inhibin alpha in serum, plasma or other biological fluids. The assay is reactive with mouse samples and uses a pre-coated 96-well microplate arranged as 12 x 8 well strips.

The workflow involves capture of Inhibin alpha by an immobilised antibody, detection with a biotinylated anti-Inhibin alpha antibody and an HRP-avidin conjugate, and visualisation using TMB substrate with absorbance read at 450 nm on a colorimetric microplate reader. The stated product range is 15.6-1000 pg/ml with a sensitivity of <6.1 pg/ml. The total assay time is 4h 30m. Kit components are stored at +4°C or -20°C as indicated and the kit should not be used past its expiration date.

Reported spike-and-recovery values are: serum n=5, range 80%-102%, average 91%; EDTA plasma n=5, range 81%-100%, average 90%; heparin plasma n=5, range 80%-89%, average 84%. Dilution linearity across 1:2, 1:4, 1:8 and 1:16 dilutions is documented for serum, EDTA plasma and heparin plasma (n=5 per matrix).