Mouse IL-1 beta ELISA Kit

Antibodies.com Mouse IL-1 beta ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse IL-1 beta in cell culture supernatant, serum and plasma. The kit falls within the Assays and Kits product category and uses a colorimetric detection format on a microplate platform, with absorbance read by a microplate reader.

Validated for mouse reactivity, the assay has shown no significant cross-reactivity and can detect both native and recombinant mouse IL-1 beta. The product range is 7.81 – 500 pg/ml, with a sensitivity of <1.03 pg/ml. Standards and samples are incubated with a biotin-linked monoclonal antibody specific for IL-1 beta, followed by streptavidin-HRP and a substrate solution that produces a colour proportional to the amount of IL-1 beta bound; the reaction is stopped with acid and the optical density is measured. Components should be stored at +4°C and the kit must not be used past its expiration date.

Across serum samples, average recovery is reported at 97%, supporting reliable quantitative measurement across the working range.