Mouse Histone Deacetylase 1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of mouse Histone Deacetylase 1 (HDAC1) in tissue homogenates, cell lysates or other biological fluids. The assay employs the sandwich enzyme immunoassay technique, in which an antibody specific for HDAC1 has been pre-coated onto a 96-well microtiter plate. Standards and test samples are added to the wells and bound HDAC1 is detected using a Biotinylated Anti-HDAC1 Antibody, followed by an HRP-Avidin conjugate and TMB substrate. The enzymatic reaction produces a coloured product, and absorbance is read at 450 nm on a colorimetric microplate reader.

The product falls within the Assays and Kits category and is reactive with mouse samples. Key performance specifications include a sensitivity of 0.21 ng/ml and an assay range of 0.781–50 ng/ml. The total assay time is 4 hours 30 minutes, and the platform is a pre-coated microplate comprising 12 x 8 well strips. Storage instructions call for keeping components at +4°C or -20°C as indicated, and the kit should not be used past its expiration date.

The kit is validated for cell lysates and tissue homogenates. Reported recovery averages across serum, EDTA plasma and heparin plasma samples are approximately 91%, 90% and 84% respectively, with individual values varying by sample dilution. Kit components include a pre-coated 96-well microplate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers.