Mouse Heat Shock Protein Beta 8 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit is designed for the in vitro quantitative determination of mouse Heat Shock Protein Beta 8 (HSP22/HSPB8) in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and relies on a biotinylated anti-HSP22/HSPB8 detection antibody with an HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

According to the manufacturer's product information, the assay range is 0.156–10 ng/ml and the sensitivity is <0.048 ng/ml, with a total assay time of 4h 30m. Reported average recovery values are 91% for serum (range 83–99%), 89% for EDTA plasma (range 80–99%), and 90% for heparin plasma (range 82–99%), each from n=5. Dilution linearity is reported for serum, EDTA plasma, and heparin plasma at 1:2, 1:4, 1:8, and 1:16 dilutions. Kit components include the pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution, and plate sealers, with storage at +4°C or -20°C as directed. An easy-to-follow protocol is provided.