Mouse Hcrt ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of mouse Prepro-Orexin/HCRT in serum, plasma, tissue homogenates, and other biological fluids. The 4-hour assay runs on a pre-coated microplate platform arranged as 12 x 8 well strips, with colorimetric detection read at 450 nm following TMB substrate development.

Stated performance includes a product range of 7.813–500 pg/mL and a sensitivity of 4.688 pg/mL, with no reported cross-reactivity to other analogs or variants. Serum and plasma samples are recommended to be diluted at least 1:2 with Sample Dilution Buffer prior to testing, with validated dilutions of 1:2 for healthy serum and healthy plasma.

Reported average recoveries (n=10) are 100% for serum (range 94–104%), 95% for EDTA plasma (range 87–103%), and 98% for heparin plasma (range 91–105%). Kit components include lyophilised standard, sample and antibody dilution buffers, biotinylated detection antibody, HRP-streptavidin conjugate, TMB substrate, stop solution, wash buffer, plate sealers, and a foil zip-sealed pouch. Most components are stored at +4°C.