Mouse Growth Differentiation Factor 15 ELISA Kit

Manufactured for Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of mouse Growth Differentiation Factor 15 (GDF15) in serum, plasma, tissue homogenates, and other biological fluids. It employs a pre-coated microplate format with colorimetric detection, read on a standard microplate reader at 450 nm.

According to the manufacturer's documentation, the assay has a sensitivity of <5.8 pg/ml and a quantitative range of 15.6–1000 pg/ml, with a total assay time of 4 hours 30 minutes. The procedure uses a biotinylated anti-GDF15 detection antibody followed by HRP-avidin and TMB substrate, producing a yellow product whose absorbance is proportional to GDF15 concentration when read against a standard curve. Kit components should be stored at +4°C or −20°C as instructed, and the kit should not be used past its expiration date.

Validation data supplied by the manufacturer includes spike-and-recovery results in serum (average 91%, range 83–100%), EDTA plasma (average 96%, range 90–103%) and heparin plasma (average 95%, range 89–102%), together with dilution linearity across 1:2 to 1:16 sample dilutions for each of these sample types.