Mouse Growth Arrest And DNA Damage Inducible Protein Gamma ELISA Kit

A sandwich ELISA from Antibodies.com is intended for the in vitro quantitative determination of mouse Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45G) in tissue homogenates, cell lysates or other biological fluids. The assay is read on a colorimetric microplate reader using a pre-coated 96-well microplate (12 x 8-well strips), and the total assay time is approximately 4 hours 30 minutes.

The kit reports a sensitivity of 0.225 ng/ml across an assay range of 0.625–40 ng/ml. Detection relies on an immobilized anti-GADD45G capture antibody, a biotinylated anti-GADD45G detection antibody and an HRP-avidin/TMB substrate system, with absorbance measured at 450 nm. Mean recovery values reported across dilutions from 1:2 to 1:16 are 91% for serum, 95% for EDTA plasma and 96% for heparin plasma. Kit components should be stored at +4°C or -20°C as instructed.