Mouse Granzyme B ELISA Kit

Antibodies.com supplies this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) for the in vitro quantitative determination of mouse Granzyme B in serum, plasma, tissue homogenates, and other biological fluids. The assay is run on a pre-coated 96-well microplate (12 x 8 well strips) using a biotinylated anti-Granzyme B detection antibody, an HRP-avidin conjugate, and TMB substrate, with absorbance read at 450 nm in a colorimetric microplate reader. The protocol takes approximately 4 hours 30 minutes, with a reported sensitivity of <5.7 pg/ml and an assay range of 15.6-1000 pg/ml. Components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.

Spike-and-recovery values reported by the manufacturer are 80%-102% for serum, 81%-100% for EDTA plasma, and 80%-89% for heparin plasma (n=5), with average recoveries of 91%, 90%, and 84% respectively, alongside linearity data for serial dilutions.