Mouse GDN ELISA Kit

This sandwich-format ELISA from Antibodies.com is a 4-hour quantitative assay designed for the in vitro determination of mouse SERPINE2/PN-1 in serum, plasma, tissue homogenates, and other biological fluids. The manufacturer description for this kit states the antibody is specific for SERPINE2/PN-1 with no reported cross-reactivity to other analogs or variants, and the assay is for use with mouse samples.

The workflow uses a pre-coated microplate with an immobilised capture antibody, followed by a biotinylated detection antibody and an HRP-Streptavidin conjugate, with a colorimetric TMB substrate read at 450 nm. The stated standard range is 6.25-400 pg/mL and sensitivity is <3.75 pg/mL. Recovery in undiluted samples (n=10) is reported as 89-104% (average 98%) for serum, 92-105% (average 97%) for EDTA plasma, and 89-101% (average 95%) for heparin plasma. Validated sample dilutions include 1:2 for healthy serum and healthy plasma.

Kit components are shipped at +4°C and stored at +4°C or -20°C as directed in the protocol manual; freeze/thaw cycles and use past the expiration date should be avoided. A third-party listing prices the Antibodies.com Mouse GDN ELISA Kit at $869.19, with shipping available to US addresses only.