Mouse Gamma-Glutamyltransferase 1 ELISA Kit

This sandwich ELISA from Antibodies.com is designed for the in vitro quantitative determination of mouse GGT1/GGT in serum, plasma, tissue homogenates, cell lysates or other biological fluids. An antibody specific for GGT1/GGT is pre-coated onto a 96-well microtiter plate; standards and samples are added, followed by a Biotinylated Anti-GGT1/GGT Antibody and an HRP-Avidin conjugate. TMB substrate produces a blue product that turns yellow upon addition of stop solution, and the density of yellow is proportional to GGT1/GGT concentration, read as O.D. absorbance at 450nm against a standard curve.

According to the manufacturer, the assay has a product range of 0.156–10 ng/ml and a sensitivity of 0.056 ng/ml (<0.056 ng/ml), with a total assay time of 4h 30m. Spike-and-recovery performance in serum averaged 91% (range 80%–102%, n=5), in EDTA plasma averaged 90% (range 81%–100%, n=5), and in heparin plasma averaged 84% (range 80%–89%, n=5). Dilution linearity at 1:2, 1:4, 1:8 and 1:16 is reported for serum, EDTA plasma and heparin plasma across the same sample set, with overlapping ranges spanning roughly 74%–108% depending on matrix and dilution.

Kit components include a pre-coated 96-well microplate (12 x 8 well strips), lyophilised standard (2 vials), detection solutions A and B, a 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, with storage distributed between +4°C and -20°C as instructed in the manual. The product is intended for research use and the protocol is described as easy to follow.