Mouse Galectin 9 ELISA Kit

From Antibodies.com, this Sandwich ELISA kit is designed for the in vitro quantitative determination of mouse Galectin 9/Gal-9 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The assay employs a pre-coated 96-well microplate configured as 12 x 8 well strips, with Biotinylated Anti-Galectin 9/Gal-9 Antibody and HRP-Avidin detection followed by TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader. The stated product range is 78.1-5000 pg/ml with a sensitivity of 29 pg/ml, and the total assay time is 4h 30m.

Validation data are reported for serum, EDTA plasma and heparin plasma. Average recovery in serum (n=5) was 91% across an 80%-102% range; EDTA plasma (n=5) showed 90% (81%-100%) and heparin plasma (n=5) showed 84% (80%-89%). Linearity of dilution was assessed at 1:2, 1:4, 1:8 and 1:16 across each matrix. Components supplied include the pre-coated microplate, lyophilized standard, Detection Solutions A and B, Wash Buffer (30X), Sample Dilution Buffer, TMB Substrate, Stop Solution and adhesive plate sealers, with storage at +4°C or -20°C as indicated for each item.