Mouse Free Fatty Acid Receptor 3 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the quantitative in vitro determination of mouse Free Fatty Acid Receptor 3 in tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and employs a biotinylated detection antibody in conjunction with an HRP-avidin conjugate. Signal is generated using a TMB substrate, producing a yellow product whose absorbance is read at 450 nm in a microplate reader.

Reported performance figures include a sensitivity of 0.053 ng/ml and a standard curve range of 0.156–10 ng/ml, with a total assay time of approximately 4 hours 30 minutes. Sample recovery data (n=5) supplied with the kit list averages of 91% in serum (range 80–102%), 90% in EDTA plasma (range 81–100%) and 84% in heparin plasma (range 80–89%), with linearity values provided across serial dilutions from 1:2 to 1:16 for each matrix. Kit components are stored at +4°C or −20°C as instructed, and the product is for research use only — not intended for diagnostic or therapeutic use.