Mouse Fractalkine ELISA Kit

Quantitative Fractalkine ELISA from Antibodies.com for measuring CX3CL1 in mouse research samples using a microplate immunoassay workflow. The kit provides reagents sufficient for 96T determinations, including pre-coated plate wells, lyophilized protein standard, biotinylated detection antibody, wash buffer concentrate, streptavidin-HRP conjugate, substrate, and stop solution. Each plate well receives 100 µL of standard or sample during the run.

Fractalkine, also known as CX3CL1 or neurotactin, is the sole member of the CX3C chemokine family, distinguished by three amino acids between its first two cysteine residues. The protein is expressed both as a transmembrane adhesion molecule on activated endothelium and neurons and as a cleaved soluble chemoattractant that signals through the CX3CR1 receptor on monocytes, NK cells, and T lymphocyte subsets. Researchers apply this microplate kit to studies of microglial activation, neuroinflammation, vascular inflammation, and peripheral leukocyte trafficking in mouse disease models.

Reagents should be refrigerated upon arrival and protected from light; reconstituted standards should be aliquoted and stored to limit freeze-thaw cycles. Absorbance is read on a standard microplate reader at the wavelength specified in the protocol. Antibodies.com confirms the assay is calibrated for mouse Fractalkine, with cross-reactivity testing against related chemokines documented in the product manual for verification before experimental use.