Mouse Forkhead Box Protein A2 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com, designed for the in vitro quantitative determination of mouse Forkhead Box Protein A2 (FOXA2) in tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated microplate format, with bound FOXA2 detected using a Biotinylated Anti-FOXA2 Antibody, an HRP-Avidin conjugate, and TMB substrate solution, with absorbance read at 450 nm on a colorimetric microplate reader.

Key performance characteristics include an assay range of 0.312-20 ng/ml and a sensitivity of <0.126 ng/ml, with a total assay time of 4 hours 30 minutes. Reported average recovery values across serum, EDTA plasma, and heparin plasma samples (n=5) were 91%, 90%, and 84% respectively, with dilution recovery ranges from 1:2 to 1:16 spanning approximately 74-108% across the same sample types.

Kit components should be stored at +4°C or -20°C as instructed, and the product is not to be used past its expiration date.