Mouse Folate Receptor 1, Adult ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is designed for the in vitro quantitative determination of mouse Folate Binding Protein (also referred to as Folate Receptor 1, Adult) in serum, plasma, tissue homogenates or other biological fluids. The assay is run on a pre-coated microplate platform (12 x 8 well strips) using a Biotinylated Anti-Folate Binding Protein/FBP Antibody, an HRP-Avidin conjugate and TMB substrate, with absorbance read at 450 nm in a colorimetric microplate reader and concentrations calculated against a standard curve.

According to the manufacturer's specification, the assay has a product range of 78.1-5000 pg/ml and a sensitivity of <31 pg/ml, with a total assay time of 4 hours 30 minutes. Reported validation data show mean recovery values of 91% for serum (range 80-102%, n=5), 90% for EDTA plasma (range 81-100%, n=5) and 84% for heparin plasma (range 80-89%, n=5). Linearity of dilution was also evaluated at 1:2, 1:4, 1:8 and 1:16 across the same sample types, with corresponding percentage ranges reported for each dilution.

The supplied protocol is described as easy to follow. Kit components should be stored at +4°C or -20°C as instructed in the manual, and the kit must not be used past its expiration date.