Mouse Ficolin 1 ELISA Kit

From Antibodies.com, the Mouse Ficolin 1 ELISA Kit (Mouse FCN1/M-Ficolin ELISA Kit) is a sandwich enzyme-linked immunosorbent assay (sELISA) for the quantitative in vitro determination of mouse Ficolin 1 in serum, plasma, tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate with a biotinylated anti-FCN1/M-Ficolin detection antibody and HRP-avidin conjugate, with signal generated using TMB substrate and read at 450 nm on a colorimetric microplate reader.

The stated assay range is 0.468-30 ng/ml with a sensitivity of <0.18 ng/ml, and the total assay time is approximately 4h 30m. Recovery has been validated in serum, EDTA plasma and heparin plasma across dilutions from 1:2 to 1:16, with mean recoveries of 91%, 90% and 84% respectively. Kit components are stored at +4°C or -20°C as instructed.