Mouse Fibrinogen Like Protein 1 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com enables the in vitro quantitative determination of mouse Fibrinogen Like Protein 1 (FGL1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The assay is run on a pre-coated 96-well microplate arranged as 12 x 8 well strips, with FGL1 captured by an immobilised antibody and detected using a biotinylated anti-FGL1 antibody followed by HRP-avidin conjugate. The enzymatic reaction is visualised with TMB substrate and quantified by measuring absorbance at 450 nm on a colorimetric microplate reader.

The assay covers a working range of 31.2-2000 pg/ml with a sensitivity of less than 11.8 pg/ml, and the full protocol can be completed in 4 hours 30 minutes. Kit components — including the pre-coated microplate, lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers — should be stored at +4°C or -20°C as instructed and used before the stated expiration date.

Validation studies in mouse samples (n=5) showed recovery of 80%-102% (average 91%) in serum, 81%-100% (average 90%) in EDTA plasma and 80%-89% (average 84%) in heparin plasma. Linearity was confirmed across serial dilutions of 1:2, 1:4, 1:8 and 1:16, with serum ranging from 74% to 107%, EDTA plasma from 79% to 108% and heparin plasma from 82% to 105% across all dilution points.