Mouse Factor Related Apoptosis Ligand ELISA Kit

From Antibodies.com, this sandwich enzyme-linked immunosorbent assay (sELISA) is intended for the quantitative determination of mouse Fas Ligand in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, or other biological fluids. The Mouse Fas Ligand ELISA Kit (also referenced as the Mouse Factor Related Apoptosis Ligand ELISA Kit) targets a protein with several synonyms, including CD178, CD95-L, FasL, FASLG, and TNFSF6.

The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with colorimetric detection by absorbance reading at 450 nm in a microplate reader. The standard curve covers a range of 0.156-10 ng/ml with a reported sensitivity of <0.060 ng/ml, and the total assay time is 4 hours 30 minutes.

Average recovery was 91% in serum (range 80-102%), 90% in EDTA plasma (81-100%), and 84% in heparin plasma (80-89%). Linearity of dilution was demonstrated at 1:2, 1:4, 1:8, and 1:16 dilutions across these sample types. Kit components include a pre-coated 96 well microplate, lyophilized standard (2 vials), detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, to be stored at +4°C or -20°C as instructed and not used past the expiration date.