Mouse F-Box And Leucine Rich Repeat Protein 3 ELISA Kit

A sandwich enzyme-linked immunosorbent assay (ELISA) from Antibodies.com is intended for the in vitro quantitative determination of mouse FBXL3 (F-Box And Leucine Rich Repeat Protein 3) in tissue homogenates or other biological fluids. The kit uses a pre-coated microplate with an antibody specific for FBXL3, and detection is achieved with a Biotinylated Anti-FBXL3 Antibody, an HRP-Avidin conjugate, and a TMB substrate. Optical density is read at 450 nm using a colorimetric microplate reader, with the FBXL3 concentration in each sample calculated by reference to a standard curve.

Stated sensitivity is 0.059 ng/ml and the assay range is 0.156–10 ng/ml. The total assay time is 4 hours 30 minutes. Kit components include a pre-coated 96-well microplate, lyophilized standard, Detection Solution A and Detection Solution B, Wash Buffer (30X), Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers, which are to be stored at +4°C or -20°C as instructed. The product is for research use only and is not intended for diagnostic or therapeutic use.