Mouse Elastin ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is designed for the quantitative determination of mouse Elastin in serum, plasma, and other biological fluids. The assay employs a pre-coated microplate with colorimetric detection read on a standard microplate reader.

Key performance specifications include a sensitivity of <0.055 ng/ml and an assay range of 0.156-10 ng/ml, with a total run time of 4 hours 30 minutes. The kit is validated for mouse serum and plasma samples. Recovery ranges are 80-102% in serum, 81-100% in EDTA plasma, and 80-89% in heparin plasma (n=5). Components are stored at +4°C or -20°C as indicated.

The assay follows a standard sandwich immunoassay protocol. Standards and samples are added to wells pre-coated with anti-Elastin antibody, followed by incubation with biotinylated anti-Elastin antibody and HRP-avidin conjugate. TMB substrate is added, and the reaction is stopped with acidic solution; the resulting yellow colour is measured at 450 nm, with intensity proportional to Elastin concentration.