Mouse Elastase 4 ELISA Kit

The Mouse Elastase 4 ELISA Kit from Antibodies.com is a quantitative two-site immunoassay purpose-built to measure Elastase 4 (ELA4) protein levels in mouse-derived samples. The 48T configuration supplies 48 test wells and accompanying reagents on a pre-coated microplate, supporting small-to-mid throughput studies of pulmonary, pancreatic, and inflammatory tissue models where neutrophil-derived serine proteases play a mechanistic role.

Each kit contains lyophilized standards, a conjugated detection antibody, wash buffer concentrate, substrate solution, and stop solution. Researchers load 100 µL of standard or diluted sample per well, generate a standard curve from the included calibrators, and quantify unknowns by interpolation. The protocol accommodates cell culture supernatants, tissue homogenates, bronchoalveolar lavage fluid, and clarified serum, with dilution guidance detailed in the package insert.

Antibodies.com supplies the Mouse Elastase 4 ELISA Kit with a lot-specific certificate of analysis and a working-reagent stability profile suited to short-term bench use; reconstituted standards should be aliquoted and frozen to limit freeze-thaw cycles. The kit is intended for research use only and is not validated for diagnostic procedures.