Mouse DNA Methyltransferase 3B ELISA Kit

From Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of mouse DNA Methyltransferase 3B (Dnmt3b) in tissue homogenates or other biological fluids. The assay employs a pre-coated 96-well microplate arranged as 12 x 8 well strips, with detection carried out colorimetrically using a microplate reader.

The assay range is 0.156–10 ng/ml, with a sensitivity of <0.047 ng/ml, and a total assay time of 4h 30m. Recovery performance has been characterised in serum, EDTA plasma, and heparin plasma across dilutions of 1:2, 1:4, 1:8, and 1:16, with reported average recoveries of 91% (serum), 95% (EDTA plasma), and 94% (heparin plasma).

Kit components are supplied for storage at +4°C or -20°C, including the pre-coated microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers.