Mouse Delta Like 1 Homolog ELISA Kit

Manufactured by Antibodies.com, this Mouse DLK-1 ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) intended for the in vitro quantitative determination of mouse Delta Like 1 Homolog (DLK-1) in serum, plasma, tissue homogenates, and other biological fluids.

The assay is run on a pre-coated microplate using a biotinylated anti-DLK-1 detection antibody and an HRP-avidin conjugate, with TMB substrate producing a colour change read at 450 nm on a colorimetric microplate reader. The quoted assay range is 1.56-100 pg/ml, sensitivity is reported as <0.63 pg/ml, and total assay time is 4 hours 30 minutes.

Reported spike-and-recovery values are 80%-102% for serum (average 91%), 81%-100% for EDTA plasma (average 90%), and 80%-89% for heparin plasma (average 84%), each from n=5. Kit reagents include pre-coated plate, lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, stored at +4°C or -20°C as indicated. The kit should not be used past its expiration date.