Mouse Defensin Beta 1 ELISA Kit

Manufactured for Antibodies.com, this Mouse Defensin Beta 1 ELISA Kit is a sandwich ELISA (sELISA) intended for the in vitro quantitative determination of mouse beta Defensin 1 in serum, plasma, tissue homogenates, and other biological fluids. The kit uses a pre-coated microplate and a colorimetric microplate reader, with absorbance measured at 450 nm.

The stated product range is 3.12-200 pg/ml with a sensitivity of 1.36 pg/ml. Total assay time is approximately 4 hours 30 minutes. Kit components should be stored at +4°C or -20°C as instructed, and the kit must not be used past its expiration date.

Validation data report average recoveries of 91% in serum (range 83%-99%, n=5), 89% in EDTA plasma (range 80%-99%, n=5), and 90% in heparin plasma (range 82%-99%, n=5). Linearity has been demonstrated in serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16.