Mouse Cystathionine Beta Synthase ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com designed for the in vitro quantitative determination of mouse Cystathionine Beta Synthase (CBS) in tissue homogenates or other biological fluids. The kit is reactive with mouse samples and falls within the Assays and Kits product category.

The assay employs the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate arranged as 12 x 8 well strips. Standards and test samples are added to the wells and the CBS present is bound by the immobilised antibody. Following a wash step, a Biotinylated Anti-CBS Antibody is added, followed by an HRP-Avidin conjugate. TMB substrate solution is then used to visualise the HRP enzymatic reaction, producing a blue product that turns yellow upon addition of acidic stop solution. Absorbance is read at 450 nm in a colorimetric microplate reader. Reported performance characteristics include a product range of 0.156-10 ng/ml, a sensitivity of <0.059 ng/ml, and a total assay time of 4h 30m. Kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date.

Spike-and-recovery testing returned ranges of 83%-99% (average 91%) for five serum samples, 80%-99% (average 89%) for five EDTA plasma samples, and 82%-99% (average 90%) for five heparin plasma samples. Dilution linearity was assessed at 1:2, 1:4, 1:8 and 1:16 dilutions, yielding the following ranges: serum (n=5) 87-109%, 74-104%, 90-94% and 86-108%; EDTA plasma (n=5) 86-101%, 90-101%, 78-110% and 84-96%; heparin plasma (n=5) 90-101%, 77-107%, 93-96% and 89-109%.