Mouse Cross Linked C-Telopeptide Of Type I Collagen ELISA Kit

A sandwich ELISA from Antibodies.com supports the in vitro quantitative measurement of mouse Cross Linked C-Telopeptide Of Type I Collagen in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids, with reactivity specific to mouse samples.

The kit uses the sandwich enzyme immunoassay technique on a 96-well microplate pre-coated with an antibody specific for the target, with a biotinylated detection antibody and an HRP-Avidin conjugate. TMB substrate yields a colour change proportional to analyte concentration, quantified by absorbance at 450 nm using a colorimetric microplate reader. The product range is 0.156-10 ng/ml with a sensitivity of 0.046 ng/ml, and total assay time is 4 hours 30 minutes.

Sample recovery has been demonstrated in serum (range 80% - 102%, average 91%, n=5), EDTA plasma (range 81% - 100%, average 90%, n=5) and heparin plasma (range 80% - 89%, average 84%, n=5). Kit components are stored at +4°C or -20°C as instructed, and the product should not be used past its expiration date.