Mouse Complement Factor H ELISA Kit

From Antibodies.com, this Mouse Complement Factor H ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) for the in vitro quantitative determination of mouse Complement Factor H in serum, plasma, or other biological fluids. The assay is run on a pre-coated microplate with colorimetric detection, and absorbance is read at 450 nm on a standard microplate reader. The full protocol can be completed in 4 hours 30 minutes.

According to the manufacturer's specifications, the assay has a detection range of 78.1-5000 pg/ml and a sensitivity of <35.5 pg/ml. Recovery of Complement Factor H has been demonstrated in serum (average 91%, range 80-102%, n=5), EDTA plasma (average 90%, range 81-100%, n=5), and heparin plasma (average 84%, range 80-89%, n=5), with dilution linearity established at 1:2, 1:4, 1:8, and 1:16 across all three sample types. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.