Mouse Complement Component 4b ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of mouse Complement C4b in serum, plasma and other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with colorimetric detection read at 450 nm on a standard microplate reader. A biotinylated anti-Complement C4b antibody and HRP-avidin conjugate are used, and TMB substrate produces a yellow product whose absorbance is proportional to the amount of Complement C4b captured in each well.

The quantitative assay has a working range of 0.625-40 ng/ml and a sensitivity of <0.245 ng/ml, with a total run time of 4 hours 30 minutes. Reported spike-and-recovery averages are 91% for serum, 94% for EDTA plasma, and 94% for heparin plasma. Dilution linearity was demonstrated across 1:2, 1:4, 1:8, and 1:16 dilutions, with ranges of 80-110% for serum, 70-108% for EDTA plasma, and 79-110% for heparin plasma. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.