Mouse Cofilin 1, Non Muscle ELISA Kit

Manufactured for Antibodies.com, this sandwich ELISA kit enables in vitro quantitative determination of mouse Cofilin (Cofilin 1, Non Muscle) in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with colorimetric detection read on a microplate reader at 450 nm.

The reported sensitivity is 0.108 ng/ml with an assay range of 0.312-20 ng/ml. Total assay time is 4 hours 30 minutes. Supplied components include a pre-coated microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, stored at +4°C or -20°C as instructed.

Recovery performance in serum (n=5) is reported as 80%-102% with an average of 91%; EDTA plasma (n=5) shows 81%-100% with an average of 90%; and heparin plasma (n=5) shows 80%-89% with an average of 84%. Linear dilution behaviour at 1:2, 1:4, 1:8 and 1:16 is reported across the same three sample types.