Mouse Cluster Of Differentiation 40 Ligand ELISA Kit

This Antibodies.com ELISA kit is a sandwich enzyme-linked immunosorbent assay designed for the in vitro quantitative determination of mouse TRAP/CD40L in serum, plasma, tissue homogenates, cell lysates, or other biological fluids. The assay uses a pre-coated microplate with a biotinylated detection antibody and HRP-avidin/TMB detection, with absorbance read at 450 nm on a colorimetric microplate reader.

The manufacturer reports an assay range of 15.6–1000 pg/ml and a sensitivity of <6.3 pg/ml, with a total assay time of 4h 30m. Reported recovery averages are 91% for serum (n=5), 96% for EDTA plasma (n=5) and 95% for heparin plasma (n=5). Dilution linearity across 1:2 to 1:16 is reported as 75–104% for serum, 83–110% for EDTA plasma, and 72–105% for heparin plasma (n=5 each).

Kit components are stored at +4°C or -20°C as instructed and the product should not be used past its expiration date.