Mouse Chromatin Assembly Factor 1, Subunit B ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of mouse p60 CAF1/MPP7 in tissue homogenates, cell lysates, or other biological fluids. The assay uses a pre-coated 96-well microplate format (12 x 8 well strips) with a colourmetric detection step read at 450 nm in a microplate reader.

The kit reports a sensitivity of <0.052 ng/ml, an assay range of 0.156–10 ng/ml, and a total assay time of 4 hours 30 minutes. Recovery testing returned averages of 91% for serum, 94% for EDTA plasma, and 95% for heparin plasma (n=5), with dilution linearity assessed from 1:2 through 1:16.

Components supplied include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. Storage temperatures range from +4°C to -20°C depending on the component, and the kit should not be used past its expiration date.