Mouse Chemokine C-C-Motif Receptor 10 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com enables the in vitro quantitative measurement of mouse Chemokine C-C-Motif Receptor 10 (CCR10) in tissue homogenates and other biological fluids. The procedure uses a pre-coated microplate together with a biotinylated anti-CCR10 detection antibody and an HRP-avidin conjugate, with signal development by TMB substrate and absorbance read at 450 nm in a colorimetric microplate reader.

Reported sensitivity is 0.051 ng/ml and the standard curve spans 0.156–10 ng/ml, with a total assay time of 4 h 30 min. Kit components are stored at +4 °C or –20 °C as instructed. In the manufacturer's validation, average spike-and-recovery values were 91% for serum (range 83–100%, n=5), 96% for EDTA plasma (range 90–103%, n=5) and 95% for heparin plasma (range 89–102%, n=5).