Mouse Chaperonin Containing TCP1, Subunit 2 ELISA Kit

From Antibodies.com, this Sandwich ELISA kit is designed for the in vitro quantitative determination of mouse CCT2 (Chaperonin Containing TCP1, Subunit 2) in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and employs the sandwich enzyme immunoassay technique with a biotinylated anti-CCT2 detection antibody and HRP-avidin conjugate, visualised via TMB substrate after addition of acidic stop solution, with absorbance read at 450 nm in a microplate reader.

Reactivity is Mouse. Reported assay range is 0.156–10 ng/ml with a sensitivity of <0.058 ng/ml, and total assay time is 4h 30m. Storage instructions specify keeping kit components at +4°C or −20°C as instructed and not using past the expiration date. Spiked recovery values reported across serum, EDTA plasma and heparin plasma samples fall between 80%–102% (serum average 91%), 81%–100% (EDTA plasma average 90%) and 80%–89% (heparin plasma average 84%), with dilution linearity data provided at 1:2, 1:4, 1:8 and 1:16 sample dilutions.